5 Parameter Blood, Glucose, Ketone, pH & Protein Test (URS) Urinalysis Reagent Strip

Testing Item: Glucose Ph Protein Ketone Blood

Dimension: 90±1(L) 5±0.2(W) 0.24-0.26(T) (mm)

1) Rapid Results: 30 seconds to 1 minutes

2) High Quality: Superior European-produced color charts provide consistent, dependable readings

3) Flexible: Available in up to 12 parameters

4) Cost Effective: 100 strips per bottle, with performance better than or equal to the current market leader at a much lower price

5) Long shelf life: Consistent Quality with 2 Year Shelf Life

Glucose

This test is based on a sequential enzyme reaction. First, glucose oxidase catalyzes the formation of gluconic acid and hydrogen peroxide from the oxidation of glucose. A second enzyme, peroxidase, catalyzes the reaction of hydrogen peroxide with potassium iodide chromogen to oxidize the chromogen to colors ranging from blue through greenish-brown, and brown to dark-brown.

Reactivity of the test decreases as the specific gravity and/or pH of urine increases, and may also vary with temperature. Ascorbic acid (more than 50 mg/dl) and ketone bodies (more than 40 mg/dl) may cause a false negative result for a specimen containing a small amount of glucose (100 mg/dl). However, the combinations of such ketone levels and low glucose levels are metabolically improbable.

pH

This test is based on double indicators (methyl red and bromothymol blue), which give a broad range of colors covering the entire urinary pH range. Colors range from orange through greenish-yellow and green to blue.

This test indicates the pH values within the range of 5 to 9.

Certain drugs, such as those used for hypertension and heart diseases (acetazolamides) may cause an alkaline urine. Excessive urine on the test strip may wash the acid buffer from the neighboring protein reagent onto the pH area and change the pH reading to an acid pH although the urine being tested may originally have been neutral or alkaline. An accurate reading may be influenced by slight variations of pigments in the urine.

Protein

This test is based on the color change of the indicator tetrabromophenol blue. A positive reaction is indicated by a color change from yellow through green and then to greenish-blue.

The minimum sensitivity of this test is 10 mg/dl of protein in urine. Highly buffered alkaline urines (pH 8.5) may give false negative results. The interpretation of results is also difficult in turbid urine specimens.

Ketones

This test is based on the reaction of acetoacetic acid in the urine with nitroprusside. The resulting color ranges from tan when no reaction takes place, to purple for a positive reaction. Normal urine specimens ordinarily yield negative results with this reagent.

False positive results may occur with highly pigmented urine specimens or those containing large amounts of levodopa metabolites.

Blood

This test is based on the pseudoperoxidase activity of hemoglobin which catalyzes the reaction of 3,3'5,5'-tetramethylbenzidine and buffered organic peroxide, 2,5-dimethylhexane-2,5-dihydroperoxide. The resulting color ranges from, greenish-yellow through bluish-green to dark blue.

A false positive test result can sometimes occur when bacteria are present in the urine. Ascorbic acid or protein may reduce the reactivity of the blood test. Strong oxidizing substances such as hypochlorites may produce a false positive result. Urine from menstruating females often, but not always, yield positive results.

This test is slightly more sensitive to free hemoglobin and myoglobin than to intact erythrocytes. The test is generally capable of detecting 0.015-mg/dl free hemoglobin or 5 to 10 intact red blood cells per ml of urine. The sensitivity may he reduced in urine with high specific gravity and ascorbic acid content. The appearance of green spots on the reagent test area indicates the presence of intact erythrocytes in the urine.

Test Procedure:

1. Remove one strip from the bottle and replace the cap immediately.

2. Immerse the reagent area of the strip in the urine specimen and take it out quickly.

3. Wipe off excess urine against the rim of the specimen container.

4. Read the test results carefully within 60 seconds in a good light and with the test area held near the
appropriate color chart on the bottle label. Changes in color that appear only along the edges of the test
pads or after moving than 2 minutes have passed are of no diagnostic significance. Results with leukocytes test portion can be read within 120 seconds.

Why It Is Done

To check for a disease or infection of the urinary tract. Symptoms of a urine infection may include colored or bad-smelling urine, pain when urinating, hard to urinate, flank pain, blood in the urine (hematuria), or fever.

To check the treatment of conditions such as diabetes, kidney stones, a urinary tract infection (UTI), high blood pressure (hypertension), or some kidney or liverdiseases.

How To Prepare

Do not eat foods that can color the urine, such as blackberries, beets, and rhubarb, before the test. Do not exercise strenuously before the test.

Tell your doctor if you are menstruating or close to starting your menstrual period. Your doctor may want to wait to do the test.

Your doctor may ask you to stop taking certain medicines that color the urine. These include vitamin B, phenazopyridine (Pyridium), rifampin, and phenytoin (Dilantin). Be sure to tell your doctor if you are taking diuretics, which may affect the test results.

Talk to your doctor any concerns you have regarding the need for the test, its risks, how it will be done, or what the results will mean. To help you understand the importance of this test, fill out the medical test information form.

Dos and don'ts:

1. Do not touch test areas of reagent strips.

2. Do not use after expiry date.

3. Do not remove desiccant(s).

4. Do not remove strip from the bottle until immediately before it is to be used for testing.

5. Replace cap immediately and tightly after removing reagent strip.

6. Store at temperature between 2°C-30°C.

7. Store only in original bottle.

8. The sample should not be more than 4 hours old at the time of testing.

9. Collect fresh urine in a clean dry container.

Urine presevatives may affect test results. Preservatives will not prevent the deterioration of ketones, bilirubin orUrobilinogen. Bacterial growth from contaminating organisms may affect glucose, ph and blood test results.

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