High Temperature Alpha-amylase/heat Stable Alpha-amylase/ heatstable alpha-amylase for brewing industry

A very cost-effective Heat-stable Alpha-amylase with the following benefits for end users:
1.Faster fermentation;
2.Reduce processing time;
3.Capable to liquefy various starch materials;
4.Robust alpha-amylase activity enables lower dosage level

DESCRIPTION

Heat-stable Alpha-amylase HS-400 is derived from a modified strain of Bacillus licheniformis using submerged fermentation and refining extraction techniques. HS-400 is an endoamylase which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.

HS-400 is suitable for starch sugar, ethanol, monosodium glutamate, beer and brewing industry.

PRODUCT CHARATERISTICS

EFFECT OF PH
HS-400 is active over a wide PH range, depending on the application. For maximum activity, the optimal PH for HS-400 is 6.0 to 6.4. At a temperature of 25° C, the stable PH range for HS-400 is 5.0 to 10.0. The exact PH optimum depends on temperature, substrate concentration , procesing time etc.

EFFECT OF TEMPERATURE

Optimum temperature of HS-400 for interval liquefaction is greater than 90° C. HS-400 liquefies starch substrates promptly at 95 to 97° C and is still highly active at 100° C. This product demonstrates exceptional thermal stability at temperatures from 105 to 110° C, keeps its stability for 5 to 7 minutes and keeps on liquefying rapidly and completely. At temperatures above 105° C, the enzyme stability decreases rapidly. The exact temperature optimum will depend on many process variables, including PH, substrate concentration and reaction time.

CONCENTRATION OF CALCIUM

The presence of calcium increases the thermal stability of HS-400. This enzyme requires a very low calcium concentration and calcium additions of 50 to 70 ppm (dry solid basis) are sufficient to ensure enzyme stability.

INHIBITORS

Copper, titanium, and cobalt ions are moderate inhibitors for HS-400. Aluminum, lead and zinc ions act as strong inhibitors.

USAGE GUIDELINES

In general, first adjust the pH to 6.0-6.4 and then add calcium (50 to 70 ppm dry solid basis) before adding HS-400. Calcium is not necessarily needed when extremely hard water (>10 German degrees) is used. After the enzyme is added, heat the slurry to liquefy.

Dosage of HS-400 is dependent on the starch substrate, application, process, substrate concentration and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product.

PACKAGING
HS-400 is packaged in 25kg, 30kg HDPE drums or 1200kg IBC totes. Customized packaging is available upon request.

STORAGE

HS-400 should be stored below 25° C in its original packaging, sealed and unopened, protected from the sun. This enzyme has been formulated for optimal stability. However, enzymes gradually lose activity over time. Extended storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement.

SAFETY & ENZYME HANDLING

Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the appropriate Material Safety Data Sheet.

CERTIFICATION

FACTORY EQUIPMENT

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